Affiliated Research Institute
Key Technology
- Isolation of various genetic materials from urine sample
- Biomarker development for non-invasive diagnosis
- Accurate diagnosis with high sensitivity/specificity
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SYBR GREEN
- As asymmetrical cyanine dye used as a nucleic acid stain
- A dsDNA binding dye used detect PCR products
- Detect any real-time PCR product w/o seq specificity.
- Optimized for a particular real-time PCR
- Specificity : Medium
- Sensitivity : Variable
- Can inhibit the PCR reaction to some extent
- Reproducibility : Medium
- Can not be used in multiplexing
- Important for gene expression analysis, DNA quantitation in pathogen detection, etc.
-
TaqMan
- The Probe Designed to increase the specificity of qPCR
- A fluorogenic probe specific to a target gene
- Only detect PCR products in a seq-specific manner
- Does not need to optimize
- Specificity : High
- Sensivity : Up to 1~10 copies
- Does inhibit the PCR reaction
- Reproducibility : High
- Allows the detection of multipie PCR products in the same sample
- Important for SNP genotyping, detection of copy no. variation such as in mutations, etc.
Technology Highlights
* Simple urine collection with a patent-registered method
- Convenient transportation of the sample in a tube containing nucleic acid preservatives
- Non-invasive liquid biopsy
- Highly sensitive diagnosis
Sample collection

- · Sample collection with a patent-registered simple method
- · Convenient transportation of the sample in a tube containing nucleic acid preservatives
Liquid Biopsy
Technology to extract and purify genetic material such as DNA and RNA from urine or saliva
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1
Extraction and concentration of organelles containing genetic material from urine, etc.
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2
Isolation of various genetic materials and development of biomarkers
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3
Quantification of biomarkers using real-time gene amplification
Development of highly accurate diagnosis method
Comparison of real-time PCR amplification methods between CYBR Green and TaqMan probes
Principle
원리
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SYBR GREEN
Denature
Polymerization
Signal detection
(Polymerization completed) -
TaqMan
Annealing
Polymerization & strand displacement
Cleavage
Signal detection
(Polymerization completed)
Comparate
비교
-
SYBR GREEN
- As asymmetrical cyanine dye used as a nucleic acid stain
- A dsDNA binding dye used detect PCR products
- Detect any real-time PCR product w/o seq specificity.
- Optimized for a particular real-time PCR
- Specificity : Medium
- Sensitivity : Variable
- Can inhibit the PCR reaction to some extent
- Reproducibility : Medium
- Can not be used in multiplexing
- Important for gene expression analysis, DNA quantitation in pathogen detection, etc.
-
TaqMan
- The Probe Designed to increase the specificity of qPCR
- A fluorogenic probe specific to a target gene
- Only detect PCR products in a seq-specific manner
- Does not need to optimize
- Specificity : High
- Sensivity : Up to 1~10 copies
- Does inhibit the PCR reaction
- Reproducibility : High
- Allows the detection of multipie PCR products in the same sample
- Important for SNP genotyping, detection of copy no. variation such as in mutations, etc.