Life Science for the Life

Affiliated Research Institute

Key Technology
  • Isolation of various genetic materials from urine sample
  • Biomarker development for non-invasive diagnosis
  • Accurate diagnosis with high sensitivity/specificity
  • SYBR GREEN
    • As asymmetrical cyanine dye used as a nucleic acid stain
    • A dsDNA binding dye used detect PCR products
    • Detect any real-time PCR product w/o seq specificity.
    • Optimized for a particular real-time PCR
    • Specificity : Medium
    • Sensitivity : Variable
    • Can inhibit the PCR reaction to some extent
    • Reproducibility : Medium
    • Can not be used in multiplexing
    • Important for gene expression analysis, DNA quantitation in pathogen detection, etc.
  • TaqMan
    • The Probe Designed to increase the specificity of qPCR
    • A fluorogenic probe specific to a target gene
    • Only detect PCR products in a seq-specific manner
    • Does not need to optimize
    • Specificity : High
    • Sensivity : Up to 1~10 copies
    • Does inhibit the PCR reaction
    • Reproducibility : High
    • Allows the detection of multipie PCR products in the same sample
    • Important for SNP genotyping, detection of copy no. variation such as in mutations, etc.
Technology Highlights
* Simple urine collection with a patent-registered method
  • Convenient transportation of the sample in a tube containing nucleic acid preservatives
  • Non-invasive liquid biopsy
  • Highly sensitive diagnosis
Sample collection
Transportation Image
  • · Sample collection with a patent-registered simple method
  • · Convenient transportation of the sample in a tube containing nucleic acid preservatives
Liquid Biopsy
Technology to extract and purify genetic material such as DNA and RNA from urine or saliva
      1

      Extraction and concentration of organelles containing genetic material from urine, etc.

      2

      Isolation of various genetic materials and development of biomarkers

      3

      Quantification of biomarkers using real-time gene amplification

Development of highly accurate diagnosis method
Comparison of real-time PCR amplification methods between CYBR Green and TaqMan probes
Principle

원리

  • SYBR GREEN

    Denature

    원리 sybr 이미지1

    Polymerization

    원리 sybr 이미지2

    Signal detection
    (Polymerization completed)

    원리 sybr 이미지3
  • TaqMan

    Annealing

    원리 taqman 이미지1

    Polymerization & strand displacement

    원리 taqman 이미지2

    Cleavage

    원리 taqman 이미지3

    Signal detection
    (Polymerization completed)

    원리 taqman 이미지4
Comparate

비교

  • SYBR GREEN
    • As asymmetrical cyanine dye used as a nucleic acid stain
    • A dsDNA binding dye used detect PCR products
    • Detect any real-time PCR product w/o seq specificity.
    • Optimized for a particular real-time PCR
    • Specificity : Medium
    • Sensitivity : Variable
    • Can inhibit the PCR reaction to some extent
    • Reproducibility : Medium
    • Can not be used in multiplexing
    • Important for gene expression analysis, DNA quantitation in pathogen detection, etc.
  • TaqMan
    • The Probe Designed to increase the specificity of qPCR
    • A fluorogenic probe specific to a target gene
    • Only detect PCR products in a seq-specific manner
    • Does not need to optimize
    • Specificity : High
    • Sensivity : Up to 1~10 copies
    • Does inhibit the PCR reaction
    • Reproducibility : High
    • Allows the detection of multipie PCR products in the same sample
    • Important for SNP genotyping, detection of copy no. variation such as in mutations, etc.